Integrative Analysis Identified BTN3A2 as a Novel Schizophrenia Risk Gene

Schizophrenia is a complex, highly heritable and heterogeneous disease with estimated heritability ranging up to 80%. It affects approximately 0.5-1% of the world population and causes significant global burdens. During the past decade, the genome-wide association studies (GWASs) have identified numerous schizophrenia-associated variants and loci in world-wide populations, but most of the risk single-nucleotide polymorphisms (SNPs) or variants identified by GWASs were located in intergenic, intronic or other non-coding regions. To identify the causal genes at the reported risk loci and to elucidate how these risk genes influence the pathogenesis of schizophrenia remains a daunting task.  

Previous studies showed that most complex disease-associated variants confer risk for the illnesses by acting as eQTL to influence gene expression in cis or in trans. By integrating a large-scale schizophrenia GWAS data and brain prefrontal cortex expression quantitative trait loci (eQTL) data, a team of scientists from Kunming Institute of Zoology, Chinese Academy of Sciences (KIZ, CAS) identified BTN3A2 as a potential schizophrenia risk gene in the Major Histocompatibility Complex (MHC) region. Result from linkage disequilibrium showed that BTN3A2 is independent of the schizophrenia risk gene C4A. Furthermore, they found that BTN3A2 expression in human brain is highest post-natally and this gene highly expressed in schizophrenia subjects compared with healthy controls. 

Evolutionary analysis found BTN3A2 is a primate specific gene. To study the function of BTN3A2 during synaptic transmission, they used cultured rat hippocampal slice as the study system and expressed BTN3A2 exogenously in CA1 neurons through biolistic transfection.  

The results showed that overexpression of BTN3A2 decreased both AMPAR and NMDAR-mediated excitatory synaptic transmission compared with the control neurons, while no effect was seen on the ratio of AMPA and NMDA EPSCs. Moreover, overexpression of BTN3A2 decreased the presynaptic release probability, most likely through its interaction with the presynaptic adhesion molecule neurexins. These results from integrative analyses and functional characterization suggest that BTN3A2 may confer risk of schizophrenia by altering excitatory synaptic function. 

This work was titled with “Identification of the primate-specific gene BTN3A2 as an additional schizophrenia risk gene in the MHC loci” and published in EBioMedicine (https://www.ebiomedicine.com/article/S2352-3964(19)30307-X/fulltext). Dr. WU Yong (KIZ, CAS) is the first author of this work. Prof. SHENG Nengyin (KIZ, CAS), Prof. LUO XiongJian (KIZ, CAS), Prof. YAO YongGang (KIZ, CAS) are the corresponding authors. This study was supported by the Strategic Priority Research Program (B) of the Chinese Academy of Sciences, the National Natural Science Foundation of China, and the Bureau of Frontier Sciences and Education, Chinese Academy of Sciences.

(By WU Yong; Editor: HE Linxi)

Contact:

HE Linxi

helinxi@mail.kiz.ac.cn 

 

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