| Tupaia GBP1 interacts with STING to initiate autophagy and restrict herpes simplex virus type 1 infection |
| 2021-12-03
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Tupaia GBP1 interacts with STING to initiate autophagy and restrict herpes simplex virus type 1 infection. J Immunol, 2021Title:Tupaia GBP1 interacts with STING to initiate autophagy and restrict herpes simplex virus type 1 infection. Author:Xie X, Han JB, Ma G, Feng XL, Li X, Zou QC, Deng ZH, Zeng J. Publication Name:J Immunol Pub Year: 2021 Doi: 10.24272/j.issn.2095-8137.2021.343 IF:5.422 Abstract: Stimulator of IFN genes (STING) is a key molecule that binds to cyclic dinucleotides produced by the cyclic GMP-AMP synthase to activate IFN expression and autophagy in the fight against microbial infection. The regulation of STING in the activation of IFN expression has been extensively reported, whereas the regulation of STING in the initiation of autophagy is still insufficiently determined. IFN-inducible guanylate-binding proteins (GBPs) are central to the cell-autonomous immunity in defending a host against viral, bacterial, and protozoan infections. In this study using the Chinese tree shrew (Tupaia belangeri chinensis), which is genetically close to primates, we found that Tupaia GBP1 (tGBP1) combines with Tupaia STING (tSTING), promotes autophagy, and moderately inhibits HSV type 1 (HSV-1) infection. The antiviral effects of tGBP1 are IFN independent. Mechanistically, tGBP1 interacted with tSTING, Tupaia sequestosome 1, and Tupaia microtubule associated protein 1 L chain 3, forming a complex which promotes autophagy in response to HSV-1 infection. This function of tGBP1 against HSV-1 infection was lost in tSTING knockout cells. Overexpression of either tSTING or its mutant tSTING-DeltaCTT that can only activate autophagy rescued the anti-HSV-1 activity of tGBP1 in tSTING knockout cells. Our study not only elucidated the underlying mechanism of tGBP1 antiviral activity against HSV-1 infection, but also uncovered the regulation of tSTING in the initiation of autophagy in response to HSV-1 infection. |
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