Speaker: Prof. Hui Yang (Institute of Neurology, Shanghai Institutes for Biological Sciences, CAS)
Title：Generation of genetically modified non-human primate and its application
Time: August 9 (Friday) 2017, 3:00pm
Venue: Laboratory of Southwestern Biodiversity, 1st floor conference room (1-24-26)
Everyone is welcome!
Genetically modified (GM) animals represent a crucial tool for understanding gene function in development and disease. The type II bacterial CRISPR/Cas system has been demonstrated as an efficient gene-targeting technology to generate mice carrying mutations and reporter. However, GM animals generated by CRISPR/Cas9 system are mosaicism, in which two or more populations of cells with different genomes are present in an individual animal. It is costly and time consuming to produce single-gene knockout animal and even more so to make double-mutant animal through the germline transmission of chimeric animals, especially for non-human primate, due to its longer sexual maturity lengths (4 to 5years) as compared to rodents. Here we optimized CRISPR/Cas9 system and obtained fully functional knockout mice for one gene or multiple genes in one step. Using this optimized CRISPR/Cas9 system, we did high-throughput screen for genes located on mouse Y chromosome. Finally, we obtain fully functional knockout monkey at first generation by this method.
Laboratory of Tumor Stem Cell
Laboratory of Medical Genetics and Evolutionary Medicine
Key Laboratory of Animal Models of Human Diseases